Safranine-o

Safranine O
Safranin is used as a counter stain in some staining protocols, coloring all cell nuclei red. This is the classic counter stain in a Gram stain. It can also be used for the detection of cartilage, mucin and mast cell granules.

CAS No. 477-73-6
C.I.No. 50240
Chemical Formula C20H19ClN4
Molecular Weight 350.85
Dye Content ~95%
Absorption Max (EtOH 50%) 530 - 534 nm
Absorptivity (1%, 1cm) > 1350 (on dry basis)
Loss On Drying (110 c) 8% max.